Part I. Handling and Analysis of RetinoidsProperties of Retinoids:Structure, Handling, and PreparationArun B. Barua and Harold C. FurrQuantitative Analyses of Naturally Occurring RetinoidsJoseph L. Napoli and Ronald L. HorstDetection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell LinesMichael WagnerPart II. Retinoid-Binding ProteinsImmunohistochemistry for CRBPs and CRABPsUlf Eriksson and Anne-Lee GustafssonWhole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic AcidSally D. LynReverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding ProteinsAndrew D. Loughney and Christopher P. F. RedfernMethods for Producing Recombinant Human Cellular Retinaldehyde-Binding ProteinJohn W. Crabb, Yang Chen, Steve Goldflam, Karen West, and James KapronExpression and Purification of CRABPs from E. coliJoseph L. Napoli, Zuzana Sperkova, Marek Sperka, and Paul D. FiorellaPurification and Fluorescent Titration of Cellular Retinol-Binding ProteinGiorgio Malpeli, Claudia Folli, Davide Cavazzini, Giovanni Sartori, and Rodolfo BerniFluorometric Titration of the CRABPsAndrew W. Norris and Ellen LiExpression and Mutagenesis of Retinol-Binding ProteinManickavasagam Sundaram, Asipu Sivaprasadarao, and John B. C. FindlayInteractions of Retinol-Binding Protein with Transthyretin and Its ReceptorAsipu Sivaprasadarao, Manickavasagam Sundaram, and John B. C. FindlayDetection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited ProteolysisRobert S. Jamison, Marcia E. Newcomer, and David E. OngMeasurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding ProteinNoa NoyUse of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene ExpressionPaul Nugent and Robert M. GreenePart III. Nuclear Retinoid ReceptorsPreparation of Polyclonal Antibodies to Retinoid ReceptorsJacqueline A. Dyck and Vickie J. LaMorteDetection of RARs and RXRs in Cells and Tissues Using Specific Ligand-Binding Assays and Ligand-Binding Immunoprecipitation TechniquesElizabeth A. AllegrettoNonisotopic In Situ Hybridization for the Detection of Nuclear Retinoid Receptor Transcripts in Tissue SectionsXiaochun Xu and Reuben LotanIn Situ Hybridization with S-Labeled Probes for Retinoid ReceptorsKaren Niederreither and Pascal DolléIsolation of Retinoid Receptors from Mammalian CellsAnn K. Daly and Christopher P. F. RedfernAnalysis of Retinoid Receptor PhosphorylationAli Tahayato, Christophe Rachez, Pierre Formstecher, and Philippe LefebvrePhotoaffinity Labeling of RARs and Mapping of Labeled Sites by an Endoproteinase Combination Techniqu Yuichi Hashimoto and Toru SasakiPCR Cloning of N-Terminal RAR Isoforms and APL-Associated PLZF-RARa Fusion ProteinsArthur ZelentRT-PCR in Diagnosis and Disease Monitoring of Acute Promyelocytic Leukemia (APL)David Grimwade, Stephen Langabeer, Kathy Howe, and Ellen SolomonA Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D ReceptorsPaul N. MacDonaldGel-Shift Analysis and Identification of RXREs and RAREs by PCR-Based SelectionMyriam I. Baes and Peter E. DeclercqIdentification and Cloning of RA-Regulated Genes by mRNA Differential DisplayJay A. White and Martin PetkovichGene Targeting of Retinoid ReceptorsDavid LohnesIndex...Mehr
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