Proprotein Convertases

Part I: Reminiscences 1. On the Discovery of Precursor Processing Donald F. Steiner 2. The Prohormone Theory and the Proprotein Convertases: It Is All About Serendipity Michel Chrétien Part II: Biochemistry and Cell Biology 3. The Proprotein Convertases, 20 Years Later Nabil G. Seidah 4. Insights from Bacterial Subtilases into the Mechanisms of Intramolecular Chaperone-Mediated Activation of Furin Ujwal Shinde and Gary Thomas 5. The Novel Role of Cathepsin L for Neuropeptide Production Illustrated by Research Strategies in Chemical Biology with Protease Gene Knockout and Expression Lydiane Funkelstein and Vivian Hook 6. In Vitro Assay for Protease Activity of Proprotein Convertase Subtilisin Kexins (PCSKs): An Overall Review of Existing and New Methodologies Ajoy Basak, Andrew Chen, Swapan Majumdar, and Heather Palmer Smith 7. Inhibitor Screening of Proprotein Convertases Using Positional Scanning Libraries Iris Lindberg and Jon R. Appel 8. Analyses of PCSK9 Post-Translational Modifications Using Time-of-Flight Mass Spectrometry Thilina Dewpura and Janice Mayne Part III: Molecular Biology and Genetics 9. The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling Philipp Stawowy and Kai Kappert 10. Identification of the Myosin Heavy Polypeptide 9 as a Downstream Effector of the Proprotein Convertases in the Human Colon Carcinoma HT-29 Cells Nathalie Scamuffa, Fabien Calvo, and Abdel-Majid Khatib 11. Regulation of 7B2 mRNA Translation: Dissecting the Role of Its 5'-Untranslated Region Haidy Tadros, Gunther Schmidt, Francine Sirois, and Majambu Mbikay 12. Analysis of Epigenetic Alterations to Proprotein Convertase Genes in Disease YangXin Fu and Mark W. Nachtigal 13. Genetic and Functional Characterization of PCSK1 Hélène Choquet, Pieter Stijnen, and John W.M. Creemers Part IV: Experimental Models 14. Prohormone Processing in Zebrafish Michael G. Morash, Kelly Soanes, and Younes Anini 15. Use of Zebrafish and Knockdown Technology to Define Proprotein Convertase Activity Babykumari P. Chitramuthu and Hugh P.J. Bennett 16. Characterization of Impaired Processing of Neuropeptides in the Brains of Endoproteases Knockout Mice Margery C. Beinfeld 17. Quantitative Peptidomics of Mice Lacking Peptide-Processing Enzymes Jonathan Wardman and Lloyd D. Fricker 18. A Proteomic Protocol to Identify Physiological Substrates of Proprotein Convertases Guiying Nie and Andrew N. Stephens 19. Neurophenotyping Genetically-Modified Mice for Social Behavior Ramona M. Rodriguiz, Jennifer S. Colvin, and William C. Wetsel... Mehr